牦牛输卵管上皮细胞和卵泡颗粒细胞的培养

分离培养牦牛输卵管上皮细胞和卵泡颗粒细胞的目的是克服体外受精中早期胚胎发育阻断,建立有利于牦牛早期胚胎体外发育的共培养体系.采集牦牛输卵管上皮细胞进行原代及传代培养,同时从卵泡液中获取颗粒细胞进行原代培养,培养过程中观察2种细胞的生长方式和形态特点.输卵管上皮细胞贴壁生长时,呈多边形,且呈单层成簇生长.培养144 h～216 h,可形成细胞单层.颗粒细胞贴壁生长时,呈现聚集生长特性,贴壁细胞形状不规则,呈放射状.培养72 h～96 h,形成颗粒细胞单层.     

Biological characteristics of JAK2 transduced CD34+ cells from cord blood during ex vivo expansion

AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34⁺ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F₂JAK2).CD34⁺cells were enriched from cord blood with a MiniMACS system.The purified CD34⁺ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34⁺ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34⁺ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33⁺,CD61⁺ and Gly-A⁺ partial positive;CD38⁺ and HLA-DR⁺ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34⁺ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy.     

渠道渐变体土方计算的精确公式

渐变体作为衔接不同断面形式的过渡段,被广泛地用于输水建筑物工程中,但其土方计算目前没有统一科学的计算方法.为此,本文列出了工程中常见的几种渐变段形式,并通过对断面函数数学特性进行分析,得妹到了常见渐变体任一断面面积函教为二次抛物线.因此,可通过起始断面面积,终止断面面积和中断面面积唯一确定任一断面面积函数,并将该函数沿渐变体长度方向积分,推导出渐变段土方计算的精确、通用计算公式.计算实例表明计算过程简单、误差小,概念清晰,可供设计人员参考.     

柴油机喷雾液滴碰撞模型的改进研究

针对用KIVA-3V软件模拟液滴碰撞的O’Rourke模型所存在的网格敏感性问题,将Nordin的MIC(Mesh Independent Collision)模型应用于液滴碰撞模拟,采用定容室对MIC模型和O’Rourke模型在不同网格喷油位置、网格密度和油滴个数条件下的喷雾形状和液滴平均半径进行了模拟比较。比较结果显示,MIC模型的稳定性、精度和可靠性等均比O’Rourke模型要好。     

饲用植物的民族植物学--内蒙古阿鲁科尔沁旗的研究案例

论述了原住民利用饲用植物资源的传统知识和经验的民族植物学研究价值，提出了我国饲用植物资源的民族植物学研究的主要内容，并以内蒙古阿鲁科尔沁旗的研究结果作了案例进行了深入的探讨。     

白花泡桐尿苷二磷酸葡萄糖焦磷酸化酶mRNA全序列克隆及序列分析

尿苷二磷酸葡萄糖焦磷酸化酶(UDP-Glucose Pyrophosphorylase UGPase)是白花泡桐(Paulownia fortunei (Seem.)Hemsl.)纤维素特异途径的关键酶.在经过对其他物种UGP mRNA序列的对比分析后,设计保守区简并引物,首先用RT-PCR方法得到413 bp UGP mRNA部分序列,之后通过RACE方法,成功克隆得到包括5'UTR和3'UTR在内的UGP全部mRNA序列,并进行了分析,所得UGP mRNA全序列共1 694个碱基,CDS共1 428个碱基(112-1539),编码氨基酸475,和其他多个物种的UGP序列比对结果显示相似度均在80％以上.     

暗期间断在植物生长发育中的应用研究(英文)

暗期间断属于光周期调控技术,被广泛应用于植物的生长发育研究。通过对暗期间断的类型、应用和机理三个方面研究成果进行了综述,其中在暗期间断类型部分总结了光质、间断暗期的次数和间隔时间方面的研究;在暗期间断应用部分总结了在植物形态发生、生理和生物化学方面的相关研究。在此基础上,提出了暗期间断在分子生物学研究及森林资源保护中的应用前景。     

中国发现光瘤姬蜂(膜翅目:姬蜂科)

报道采自河南陕县的姬蜂科中国1新记录属、新记录种——毛光瘤姬蜂Liotryphon crassiseta（Thomson，1877）。介绍了该属的主要鉴别特征和该种的形态特征、寄主、分布。     

烘烤工艺对烟叶色素及主要化学成分的影响

以烤烟G80为材料,研究了不同烘烤工艺对叶片色素含量及主要化学成分的影响.结果表明:中温中湿烘烤工艺有利于烟叶内部物质的充分转化,促进上等优质烟叶的形成.     

Effects of STK15 overexpression on growth of human esophageal carcinoma cell line KYSE150

AIM:To investigate the effects of serine/threonine kinase 15 (STK15) overexpression on the growth of human esophageal squamous-cell carcinoma (ESCC) cell line KYSE150. METHODS:Recombinant pEGFP-C1-STK15 expression vector was transfected into KYSE150 cells using LipofectamineTM 2000 and the expression of STK15 was detected by fluorescence microscopy and Western blotting. The proliferation of the cells in vitro was measured by MTT assay. The cell cycle distribution and apoptosis were detected by flow cytometry. The proliferation of the cells in vivo was measured by tumorigenicity experiment in nude mice. RESULTS:After recombinant pEGFP-C1-STK15 expression vector was stably transfected into KYSE150 cells, GFP-STK15 fusion protein localized to centrosome and spindle. The STK15-overexpressing colonies were further confirmed by Western blotting. MTT assay showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). Flow cytometry analysis showed that the percentage of the cells in G0/G1 phase and the cell apoptosis in STK15 overexpression group were decreased compared with control group (P<0.01). The tumorigenicity experiment in nude mice showed that the proliferation of the cells in STK15 overexpression group was increased compared with control group (P<0.01). CONCLUSION: Overexpression of STK15 in human ESCC KYSE150 cells promotes the cell growth in vitro and in vivo, indicating that STK15 may serve as a novel therapeutic target for esophageal carcinoma.